Journal of Advances in Environmental Health Research

Journal of Advances in Environmental Health Research

Chromium bioremediation by Alcaligenes sp. strain newly isolated from chromite mine of Sabzevar

Document Type : Original Article

Authors
Mohsen Mesbahi-Nowrouzi 1
Nasrin Mollania 1
Khosro Khajeh 2
1 Department of Biology, Faculty of Basic Sciences, Hakim Sabzevari University, Sabzevar, Iran
2 Department of Biochemistry and Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
10.22102/jaehr.2018.82265.1014
Abstract
In this work, CKCr-6A strain was found to be highly resistant to some toxic heavy metals such as Cr+6, Cr+3, Cu+2, Co+2, Cd+2, Pb+2, Hg+2, U+6, tellurium, and selenite. Herein, high chromate tolerance of an isolated strain is reported with a high minimum inhibitory concentration value of 80,000 mg/L and the effective parameters (pH, temperature, shaking, and glucose concentration) were selected for Cr(VI) removal by this isolated strain. Cr(VI) elimination by the target strain increased with glucose addition to the culture medium. We optimized the possible parameters and their interactions using design experimental software. After optimization, this strain showed high efficiency in detoxifying chromate; this could reduce up to 100 mg/L of Cr(VI) to Cr(III) over 3 h. The CKCr-6A strain exhibited ability to in vitro reduction after 3 h and repeated removing of Cr(VI) without any amendment of nutrients, suggesting its possible application in continuous bioremediation.
Keywords
Bioremediation
Carcinogenic Cr(VI)
Chromium reduction
Reductase
Response Surface Methodology
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Journal of Advances in Environmental Health Research
Volume 5, Issue 4
Autumn 2017
Pages 220-232