Adherence patterns of diarrheagenic Escherichia coli strains isolated from children with gastrointestinal diarrhea and matched controls in a Hep-2 cell adhesion assay

Document Type : Original Article


1 Dietary Supplements and Probiotic Research Center AND Department of Biochemistry, Genetic, and Nutrition, Alborz University of Medical Sciences, Karaj, Iran

2 Department of Microbiology and Immunology, Alborz University of Medical Sciences, Karaj, Iran

3 Dietary Supplements and Probiotic Research Center Alborz University of Medical Sciences, Karaj, Iran

4 Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran


In the present study, carbon species including organic carbon (OC), elemental carbon (EC), and water-soluble organic carbon (WSOC) concentration in PM2.5 were assessed at an urban site of Tehran, Iran during March to June 2014. The PM2.5 samples were collected using an frmOMNITM Ambient Air Sampler. Thermal gravimetric analysis (TGA) was used to analyze OC and EC. The results showed that PM2.5 concentrations varied from 14.32 to 74.45 μg/m3 with an average value of 41.39 μg/m3. The results also showed that carbon species varied from 5.52 to 23.21 (15.35 ± 6.05) μg/m3 for OC and 1.03 to 4.16 (2.25 ± 0.65) μg/m3 for EC. As the findings indicated, the mean PM2.5 level in the sampling area was higher than the annual average determined by the United States Environmental Protection Agency (EPA) as the ambient air quality standard. On average, carbon species (OC, EC, and WSOC) account for almost 60% of PM2.5 mass in the atmospheric outflow from a downwind site. OC and EC concentrations in atmospheric PM2.5 collected at the sampling site were lower than the values reported for other urban areas with high or medium vehicular traffic and/or industrial sources. Moreover, the results obtained in this research can provide a valuable data base for health risk evaluation of the local residents and prioritization of control actions. 


1. Nguyen TV, Le Van P, Le Huy C, Gia KN, Weintraub A. Detection and characterization of diarrheagenic Escherichia coli from young children in Hanoi, Vietnam. J Clin Microbiol 2005; 43(2): 755-60.
2. Levert M, Zamfir O, Clermont O, Bouvet O, Lespinats S, Hipeaux MC, et al. Molecular and evolutionary bases of within-patient genotypic and phenotypic diversity in Escherichia coli extraintestinal infections. PLoS Pathog 2010; 6(9): e1001125.
3. Mansan-Almeida R, Pereira AL, Giugliano LG. Diffusely adherent Escherichia coli strains isolated from children and adults constitute two different populations. BMC Microbiol 2013; 13: 22.
4. Lu L, Walker WA. Pathologic and physiologic interactions of bacteria with the gastrointestinal epithelium. Am J Clin Nutr 2001; 73(6): 1124S-30S.
5. Kalantar E, Solatni J, Khosravi B, Salehi A. Frequency of E. coli pathotypes in acute diarrhea of children and its related factorsat Beassat hospital, Sanandaj. Asian Pac J Trop Med 2009; 2(4): 64-6.
6. Jenkins C, Tembo M, Chart H, Cheasty T, Willshaw GA, Phillips AD, et al. Detection of enteroaggregative Escherichia coli in faecal samples from patients in the community with diarrhoea. J Med Microbiol 2006; 55(Pt 11): 1493-7.
7. Jafari A, Aslani MM, Bouzari S. Escherichia coli: a brief review of diarrheagenic pathotypes and their role in diarrheal diseases in Iran. Iran J Microbiol 2012; 4(3): 102-17.
8. Ochoa TJ, Contreras CA. Enteropathogenic escherichia coli infection in children. Curr Opin
Infect Dis 2011; 24(5): 478-83.
9. Belanger L, Garenaux A, Harel J, Boulianne M, Nadeau E, Dozois CM. Escherichia coli from animal reservoirs as a potential source of human extraintestinal pathogenic E. coli. FEMS Immunol Med Microbiol 2011; 62(1): 1-10.
10. Abe CM, Trabulsi LR, Blanco J, Blanco M, Dahbi G, Blanco JE, et al. Virulence features of atypical enteropathogenic Escherichia coli identified by the eae(+) EAF-negative stx(-) genetic profile. Diagn Microbiol Infect Dis 2009; 64(4): 357-65.
11. Guerrant RL, Hughes JM, Lima NL, Crane J. Diarrhea in developed and developing countries: magnitude, special settings, and etiologies. Rev Infect Dis 1990; 12 Suppl 1: S41-S50.
12. Nakhjavani FA, Emaneini M, Hosseini H, Iman-Eini H, Aligholi M, Jabalameli F, et al. Molecular analysis of typical and atypical enteropathogenic Escherichia coli (EPEC) isolated from children with diarrhoea. J Med Microbiol 2013; 62(Pt 2): 191-5.
13. Bonkoungou IJ, Haukka K, Osterblad M, Hakanen AJ, Traore AS, Barro N, et al. Bacterial and viral etiology of childhood diarrhea in Ouagadougou, Burkina Faso. BMC Pediatr 2013; 13: 36.
14. Lozer DM, Souza TB, Monfardini MV, Vicentini F, Kitagawa SS, Scaletsky IC, et al. Genotypic and phenotypic analysis of diarrheagenic Escherichia coli strains isolated from Brazilian children living in low socioeconomic level communities. BMC Infect Dis 2013; 13: 418.
15. Polotsky Y, Nataro JP, Kotler D, Barrett TJ, Orenstein JM. HEp-2 cell adherence patterns, serotyping, and DNA analysis of Escherichia coli isolates from eight patients with AIDS and chronic diarrhea. J Clin Microbiol 1997; 35(8): 1952-8.
16. Adlerberth I, Jalil F, Carlsson B, Mellander L, Hanson LA, Larsson P, et al. High turnover rate of Escherichia coli strains in the intestinal flora of infants in Pakistan. Epidemiol Infect 1998; 121(3): 587-98.
17. Ghaderian MH, Mehrabani Natanzi M, Goudarzvand M, Khodaii Z. Probiotic therapy, what is the most effective method for host protection against enteric pathogen. International Journal of Enteric Pathogens 2013; 1(2): YY-ZZ.
18. Levican A, Alkeskas A, Gunter C, Forsythe SJ, Figueras MJ. Adherence to and invasion of human intestinal cells by Arcobacter species and their virulence genotypes. Appl Environ Microbiol 2013; 79(16): 4951-7.