Removal of nickel and total chromium using Escherichia coli biofilm supported on clinoptilolite


1 Kurdistan Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran

2 Deputy of Research, Kurdistan University of Medical Sciences, Sanandaj, Iran


Biofilm is communities of microorganisms attached to the surface and is able to concentrate metal species within their cell structure. Therefore, the aim of this study was to produce Escherichia coli biofilm on zeolite (clinoptilolite) and evaluate its ability for nickel (Ni) and chromium (Cr) adsorption from aqueous solutions. A laboratory-scale batch model was used for biodsorption assay. The effect of initial metal concentrations and pH on the removal efficiency was studied. Two isotherm equations were used for analyzing the experimental data. The results showed that Ni uptake by biofilm were higher than Cr. The biosorption process was best described by the Langmuir model. Fourier transform infrared confirmed that there are some functional groups on the biomass surface that may interact with the metal ions. It is concluded that the biofilm is very promising for the removal of metal ions from aqueous solution and hence may be encourage the utilization of biofilm in environmental applications.  


  1. Maleki A, Zarasvand MA. Heavy metals in selected edible vegetables and estimation of their daily intake in Sanandaj, Iran. Southeast Asian J Trop Med Public Health 2008; 39(2): 335-40.
  2. Maleki A, Mahvi AH, Zazouli MA, Izanloo H, Barati AH. Aqueous Cadmium Removal by Adsorption on Barley Hull and Barley Hull Ash. Asian J Chem 2011; 23(3): 1373-6.
  3. Quintelas C, Rocha Z, Silva B, Fonseca B, Figueiredo H, Tavares T. Biosorptive performance of an Escherichia coli biofilm supported on zeolite NaY for the removal of Cr(VI), Cd(II), Fe(III) and Ni(II). Chemical Engineering Journal 2009; 152(1): 110-5.
  4. Maleki A, Erfan MB, Mohammadi AS, Ebrahimi R. Application of commercial powdered activated carbon for adsorption of carbolic acid in aqueous solution. Pak J Biol Sci 2007; 10(14): 2348-52.
  5. Quintelas C, Fernandes B, Castro J, Figueiredo H, Tavares T. Biosorption of Cr(VI) by a Bacillus coagulans biofilm supported on granular activated carbon (GAC). Chemical Engineering Journal 2008; 136: 195-203.
  6. Lameiras S, Quintelas C, Tavares T. Biosorption of Cr (VI) using a bacterial biofilm supported on granular activated carbon and on zeolite. Bioresour Technol 2008; 99(4): 801-6.
  7. Quintelas C, Fonseca B, Silva B, Figueiredo H, Tavares T. Treatment of chromium(VI) solutions in a pilot-scale bioreactor through a biofilm of Arthrobacter viscosus supported on GAC. Bioresour Technol 2009; 100(1): 220-6.
  8. Comte S, Guibaud G, Baudu M. Biosorption properties of extracellular polymeric substances (EPS) towards Cd, Cu and Pb for different pH values. J Hazard Mater 2008; 151(1): 185-93.
  9. Eboigbodin KE, Biggs CA. Characterization of the extracellular polymeric substances produced by Escherichia coli using infrared spectroscopic, proteomic, and aggregation studies. Biomacromolecules 2008; 9(2): 686-95.
  10. Wang W, Wang W, Zhang X, Wang D. Adsorption of p-chlorophenol by biofilm components. Water Res 2002; 36(3): 551-60.
  11. Leung S, Barrington S, Wan Y, Zhao X, El-Husseini B. Zeolite (clinoptilolite) as feed additive to reduce manure mineral content. Bioresour Technol 2007; 98(17): 3309-16.
  12. Kocaoba S, Orhan Y+, Akyuz T. Kinetics and equilibrium studies of heavy metal ions removalby use of natural zeolite. Desalination 2007; 214(1-3): 1-10.
  13. Castaldi P, Santona L, Enzo S, Melis P. Sorption processes and XRD analysis of a natural zeolite exchanged with Pb(2+), Cd(2+) and Zn(2+) cations. J Hazard Mater 2008; 156(1-3): 428-34.
  14. Wingenfelder U, Nowack B, Furrer G, Schulin R. Adsorption of Pb and Cd by amine-modified zeolite. Water Res 2005; 39(14): 3287-97.
  15. Dang VB, Doan HD, Dang-Vu T, Lohi A. Equilibrium and kinetics of biosorption of cadmium(II) and copper(II) ions by wheat straw. Bioresour Technol 2009; 100(1): 211-9.
  16. Sari A TMUOSM. Biosorption of Pb(II) and Ni(II) from aqueous solution by lichen (Cladonia furcata) biomass. Biochemical Engineering Journal 2007; 37(2): 151-8.
  17. Bhattacharyya KG, Gupta SS. Influence of acid activation on adsorption of Ni(II) and Cu(II) on kaolinite and montmorillonite: Kinetic and thermodynamic study. Chemical Engineering Journal 2008; 136(1): 1-13.
  18. Carvalho WA, Vignado C, Fontana J. Ni(II) removal from aqueous effluents by silylated clays. J Hazard Mater 2008; 153(3): 1240-7.
  19. Vijayaraghavan K, Lee MW, Yun YS. Evaluation of fermentation waste (Corynebacterium glutamicum) as a biosorbent for the treatment of nickel(II)-bearing solutions. Biochemical Engineering Journal 2008; 41(3): 228-33.
  20. Quintelas C, Rocha Z+, Silva B, Fonseca B, Figueiredo H, Tavares T. Removal of Cd(II), Cr(VI), Fe(III) and Ni(II) from aqueous solutions by an E. coli biofilm supported on kaolin. Chemical Engineering Journal 2009; 149(1-3): 319-24.
  21. Wan Ngah WS, Hanafiah MAKM. Adsorption of copper on rubber (Hevea brasiliensis) leaf powder: Kinetic, equilibrium and thermodynamic studies. Biochemical Engineering Journal 2008; 39(3): 521-30.
  22. Horsfall M, Ogban F, Akporhonor EE. Sorption of chromium (VI) from aqueous solution by cassava (Manihot sculenta Cranz.) waste biomass. Chem Biodivers 2006; 3(2): 161-74.
  23. Akhtar K, Akhtar MW, Khalid AM. Removal and recovery of uranium from aqueous solutions by Trichoderma harzianum. Water Res 2007; 41(6): 1366-78.
  24. Pradhan S, Singh S, Rai LC. Characterization of various functional groups present in the capsule of Microcystis and study of their role in biosorption of Fe, Ni and Cr. Bioresour Technol 2007; 98(3): 595-601.
  25. Barros MA, Machado N, Sousa-Aguiar E, Alves F, Pedroza S. Removal of Cr3+ from industrial and synthetic wastewater by naturally occurring clinoptilolite. In: Blanco J, Avila P, Editors. Catalizadores y adsorbentes para la protecci?n ambiental en la Regi?n Iberoamericana. Madrid, Spain: Cyted; 2001. p. 219-24. [In Spanish].
  26. Volesky B. Biosorption and me. Water Res 2007; 41(18): 4017-29.
  27. Pavan FA, Mazzocato AC, Jacques RA, Dias SLP. Ponkan peel: A potential biosorbent for removal of Pb(II) ions from aqueous solution. Biochemical Engineering Journal 2008; 40(2): 357-62.