Phytochemical Measurements and Anti-microbial Evaluation of Neem Seed Extract

Document Type : Original Article

Authors

1 Environmental Engineering, Jimma Technology Institute, Jimma University, Jimma City, Ethiopia

2 Process Engineering, Jimma Technology Institute, Jimma University, Jimma City, Ethiopia

10.34172/jaehr.1313

Abstract

Background: The plants can introduce a wide range of phytochemical components which are considered as secondary metabolites. As antioxidants, phytochemicals prevent cell damages which are usually caused by free radicals especially those which are associated with heart disease and cancer. Neem seed has high potential of phytochemicals which is used as traditional medicinal. This study aimed to evaluate bioactive and phytochemical extract of Neem seed.
Methods: The antimicrobial activities and bioactive compounds of Neem seed extracted with distilled water were investigated in this study. Bioactive components of Neem seed were examined by agar well diffusion method.
Results: Tannins, quinones and phenols were the highest phytochemical in Neem seed bioactive extract. Anthocyanin, terpenoids, saponins and glycosides were in medium levels. Also, alkaloids and flavonoids were in low level. At the lowest concentration of bioactive (10 mg/mL), Bacillus subtilis (G + ) was the best controllable compared to others. The optimum concentration of the selected pathogenic species was 55 mg/mL at which the Salmonella typhimurium (G-) was highly controllable. Staphylococcus aureus (G + ), Bacillus subtilis (G + ), and Escherichia coli (G-) were 40 mm, 38 mm and 34 mm, respectively.
Conclusion: Tannin and phenol were the highest phytochemical concentration in the Neem seed. In general the Neem seed extract seems to be antioxidants and antimicrobials with effective control area.

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  1. McEwen SA, Collignon PJ. Antimicrobial resistance: a one health perspective. Microbiol Spectr. 2018;6(2). doi: 1128/ microbiolspec.ARBA-0009-2017.
  2. Singh S, Singh DB, Singh S, Shukla R, Ramteke PW, Misra K. Exploring medicinal plant legacy for drug discovery in post-genomic era. Proc Natl Acad Sci India Sect B Biol Sci. 2019;89(4):1141-51. doi: 1007/s40011-018-1013-x.
  3. Pavela R. History, presence and perspective of using plant extracts as commercial botanical insecticides and farm products for protection against insects–a review. Plant Prot Sci. 2016;52(4):229-41.
  4. Ghosh A, Chowdhury N, Chandra G. Plant extracts as potential mosquito larvicides. Indian J Med Res. 2012;135(5):581-98.
  5. Gupta RK, Patel AK, Shah N, Chaudhary AK, Jha UK, Yadav UC, et al. Oxidative stress and antioxidants in disease and cancer: a review. Asian Pac J Cancer Prev. 2014;15(11):4405- 9. doi: 7314/apjcp.2014.15.11.4405.
  6. Sarker U, Oba S. Drought stress effects on growth, ROS markers, compatible solutes, phenolics, flavonoids, and antioxidant activity in Amaranthus tricolor. Appl Biochem Biotechnol. 2018;186(4):999-1016. doi: 1007/s12010- 018-2784-5.
  7. Sood A, Lang DK, Kaur R, Saini B, Arora S. Relevance of aromatase inhibitors in breast cancer treatment. Curr Top Med Chem. 2021;21(15):1319-36. doi: 2174/15680266216662 10701143445.
  8. Jasek K, Kubatka P, Samec M, Liskova A, Smejkal K, Vybohova D, et al. DNA methylation status in cancer disease: modulations by plant-derived natural compounds and dietary interventions. Biomolecules. 2019;9(7):289. doi: 3390/biom9070289.
  9. Křížová L, Dadáková K, Kašparovská J, Kašparovský T. Isoflavones. Molecules. 2019;24(6):1076. doi: 3390/ molecules24061076.
  10. Klančnik A, Šimunović K, Sterniša M, Ramić D, Smole Možina S, Bucar F. Anti-adhesion activity of phytochemicals to prevent Campylobacter jejuni biofilm formation on abiotic surfaces. Phytochem Rev. 2021;20(1):55-84. doi: 1007/s11101-020- 09669-6.
  11. Russell A. Anthropology of Tobacco: Ethnographic Adventures in Non-Human Worlds. Taylor & Francis; 2019.
  12. Aziz MA. Qualitative phytochemical screening and evaluation of anti-inflammatory, analgesic and antipyretic activities of Microcos paniculata barks and fruits. J Integr Med. 2015;13(3):173-84. doi: 1016/s2095-4964(15)60179-0.
  13. Pathan AA, Shah RH, Vaghela HM. The Biogenic Synthesis of Au, Pd and Pt Nanoparticles AND Its Medicinal Applications: A Review. Cambridge Scholars Publishing; 2018.
  14. Chava VR, Manjunath SM, Rajanikanth AV, Sridevi N. The efficacy of Neem extract on four microorganisms responsible for causing dental caries viz Streptococcus mutans, Streptococcus salivarius, Streptococcus mitis and Streptococcus sanguis: an in vitro study. J Contemp Dent Pract. 2012;13(6):769-72. doi: 5005/jp-journals-10024-1227.
  15. Babatunde DE, Otusemade GO, Ojewumi ME, Agboola O, Oyeniyi E, Deborah Akinlabu K. Antimicrobial activity and phytochemical screening of Neem leaves and lemon grass essential oil extracts. Int J Mech Eng Technol. 2019;10(3):882- 9.
  16. Jain A, Joshi A, Joshi J, Tatawat M, Saeed S, Telang S, et al. Comparative study of phytochemical screening and antibacterial activity of four medicinal plants. J Med Plants. 2019;7(4):81-9.
  17. Pandey A, Tripathi S. Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. J Pharmacogn Phytochem. 2014;2(5):115-9.
  18. Moonmun D, Majumder R, Lopamudra A. Quantitative phytochemical estimation and evaluation of antioxidant and antibacterial activity of methanol and ethanol extracts of Heliconia rostrata. Indian J Pharm Sci. 2017;79(1):79-90.
  19. Sharma T, Sidhu MC. Cytomorphological and preliminary phytochemical screening of Eclipta alba (L.) Hassk. Int J Green Pharm. 2017;11(1):S23-32. doi: 22377/ijgp.v11i01.855.
  20. Kumar RS, Balasubramanian P, Govindaraj P, Krishnaveni T. Preliminary studies on phytochemicals and antimicrobial activity of solvent extracts of Coriandrum sativum roots (coriander). J Pharmacogn Phytochem. 2014;2(6):74-8.
  21. Varadharajan V, Janarthanan UK, Krishnamurthy V. Physicochemical, phytochemical screening and profiling of secondary metabolites of Annona squamosa leaf extract. World J Pharm Res. 2012;1(4):1143-64.
  22. Sinaga M, Ganesan K, Nair SK, Gani SB. Preliminary phytochemical analysis and in vitro antibacterial activity of bark and seeds of Ethiopian Neem (Azadirachta indica Juss). World J Pharm Pharm Sci. 2016;5(4):1714-23.
  23. Pereira V, Dias C, Vasconcelos MC, Rosa E, Saavedra MJ. Antibacterial activity and synergistic effects between Eucalyptus globulus leaf residues (essential oils and extracts) and antibiotics against several isolates of respiratory tract infections (Pseudomonas aeruginosa). Ind Crops Prod. 2014;52:1-7. doi: 1016/j.indcrop.2013.09.032.