Journal of Advances in Environmental Health Research

Journal of Advances in Environmental Health Research

Investigating the Growth Rate of Tetraselmis Algae and its Effect on Nitrogen Removal From Aquaculture Wastewater

Document Type : Original Article

Authors
Mahsa Ghadamzadeh 1
Reza Jalilzadeh Yengejeh 1
Narges Javadzadeh 2
Azita Koushafar 3
Sina Attarroshan 3
1 Department of Environmental Engineering, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran
2 Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran
3 Department of Environment, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran
10.34172/jaehr.1314
Abstract
Background: This research investigated the ability of Tetraselmis microalgae to remove nitrates from aquaculture centers’ wastewater. Specifically, this study aimed to investigate the amount of nitrogen reduction by Tetraselmis microalgae under laboratory conditions. Therefore, algal density, chlorophyll a, specific growth rate, nitrate, and ammonia levels were investigated.
Methods: Tetraselmis microalgae was planted under laboratory conditions with constant density of 2000 mL including 1900 mL of distilled water and 100 mL of stock to investigate the refining effects for 15 days. Along to the test steps, the concentration of the mentioned factors was determined using standard methods and ultraviolet spectrophotometer.
Results: The results showed that algal density, chlorophyll concentration, and specific growth rate performed better than others in the pilot plants containing wastewater. Also, use of the studied algae caused a 50% reduction in ammonia and 80% in nitrate from aquaculture center wastewater. The data modeling showed that the above- entioned microalgae were effective in reduction of nitrogen from aquaculture center wastewater as much as 75%.
Conclusion: The results showed that the species can reduce organic substances in the wastewater of aquaculture center and their discharge into the environment.
Keywords
Wastewater treatment
Tetraselmis algae
Purification
Removal of nitrates

Subjects

  1. Kurniawan S, Novarini, Yuliwati E, Ariyanto E, Morsin M, Sanudin R, et al. Greywater treatment technologies for aquaculture safety: review. J King Saud Univ Eng Sci. 2023;35(5):327-34. doi: 1016/j.jksues.2021.03.014.
  2. Ahmad AL, Chin JY, Mohd Harun MH, Low SC. Environmental impacts and imperative technologies towards sustainable treatment of aquaculture wastewater: a review. J Water Process Eng. 2022;46:102553. doi: 1016/j.jwpe.2021.102553.
  3. Shahandeh N, Jalilzadeh Yengejeh R. Efficiency of SBR process with a six sequence aerobic-anaerobic cycle for phosphorus and organic material removal from municipal wastewater. Iran J Toxicol. 2018;12(2):27-32. doi: 29252/arakmu.12.2.27.
  4. Amini Fard F, Jalilzadeh Yengejeh R, Ghaeni M. Efficiency of microalgae Scenedesmus in the removal of nitrogen from municipal wastewaters. Iran J Toxicol. 2019;13(2):1-6. doi: 32598/ijt.13.2.483.2.
  5. Tadros HR, Salem DM, Moawad MN. Evaluation of the antifouling efficacy of biogenic constituents of some algal species. Prog Org Coats. 2021;158:106354. doi: 1016/j. porgcoat.2021.106354.
  6. Rinne H, Kostamo K. Distribution and species composition of red algal communities in the northern Baltic Sea. Estuar Coast Shelf Sci. 2022;269:107806. doi: 1016/j.ecss.2022.107806.
  7. Adejimi OE, Ignat T, Sadhasivam G, Zakin V, Schmilovitch Z, Shapiro OH. Low-resolution Raman spectroscopy for the detection of contaminant species in algal bioreactors. Sci Total Environ. 2022;809:151138. doi: 1016/j. scitotenv.2021.151138.
  8. Guo J, Ma Y, Lee JH. Real-time automated identification of algal bloom species for fisheries management in subtropical coastal waters. J. Hydro Environ Res. 2021;36:1-32. doi: 1016/j.jher.2021.03.002.
  9. Mohseni A, Fan L, Roddick FA. Impact of microalgae species and solution salinity on algal treatment of wastewater reverse osmosis concentrate. Chemosphere. 2021;285:131487. doi: 1016/j.chemosphere.2021.131487.
  10. Azarm L, Javadzadeh N, Jalilzadeh R. Investigation of Chlorella vulgaris capacity in absorption of nitrate and phosphate from wastewater of fish farming pool in Khuzestan province. J Anim Environ. 2020;12(2):291-8. doi: 22034/aej.2020.107264.
  11. Heidari Farsani M, Jalilzadeh Yengejeh R, Hajiseyed Mirzahosseini A, Monavari M, Hassani AH, Mengelizadeh N. Study of the performance of bench-scale electro-membranes bioreactor in leachate treatment. Adv Environ Technol. 2021;7(3):209-20. doi: 22104/aet.2022.5266.1426.
  12. Ghosh S, Sikdar J, Mukherjee SK, Hossain ST. Use of bioprospecting approach to reveal the potential of various algal species for value added compounds. In: Shah M, Rodriguez- Couto S, De La Cruz CB, Biswas J, eds. An Integration of Phycoremediation Processes in Wastewater Treatment. Elsevier; 2022. p. 271-88. doi: 1016/b978-0-12-823499- 0.00013-4.
  13. Tsarpali M, Arora N, Kuhn JN, Philippidis GP. Beneficial use of the aqueous phase generated during hydrothermal carbonization of algae as nutrient source for algae cultivation. Algal Res. 2021;60:102485. doi: 1016/j.algal.2021.102485.
  14. Harmon VL, Wolfrum E, Knoshaug EP, Davis R, Laurens LML, Pienkos PT, et al. Reliability metrics and their management implications for open pond algae cultivation. Algal Res. 2021;55:102249. doi: 1016/j.algal.2021.102249.
  15. Khatoon H, Penz Penz K, Banerjee S, Redwanur Rahman M, Mahmud Minhaz T, Islam Z, et al. Immobilized Tetraselmis for reducing nitrogenous and phosphorous compounds from aquaculture wastewater. Bioresour Technol. 2021;338:125529. doi: 10.1016/j.biortech.2021.125529.
  16. Beyer CP, Gómez S, Lara G, Monsalve JP, Orellana J, Hurtado CF. Sarcocornia neei: a novel halophyte species for bioremediation of marine aquaculture wastewater and production diversification in integrated systems. Aquaculture. 2021;543:736971. doi: 1016/j.aquaculture.2021.736971.
  17. Ebadi M, Asareh A, Jalilzadeh Yengejeh R, Hedayat N. Investigation of electro-coagulation process for phosphate and nitrate removal from sugarcane wastewaters. Iran J Toxicol. 2021;15(1):19-26. doi: 32598/ijt.15.1.483.4.
  18. Lo E, Arora N, Philippidis GP. Physiological insights into enhanced lipid accumulation and temperature tolerance by Tetraselmis suecica ultraviolet mutants. Sci Total Environ. 2022;839:156361. doi: 1016/j.scitotenv.2022.156361.
  19. Hu N, Xu Y, Sun C, Zhu L, Sun S, Zhao Y, et al. Removal of atrazine in catalytic degradation solutions by microalgae Chlorella and evaluation of toxicity of degradation products via algal growth and photosynthetic activity. Ecotoxicol Environ Saf. 2021;207:111546. doi: 10.1016/j.ecoenv.2020.111546.
  20. Goswami RK, Agrawal K, Verma P. An exploration of natural synergy using microalgae for the remediation of pharmaceuticals and xenobiotics in wastewater. Algal Res. 2022;64:102703. doi: 1016/j.algal.2022.102703.
  21. Katiyar R, Gurjar BR, Kumar A, Bharti RK. An integrated approach for phycoremediation of municipal wastewater and production of sustainable transportation fuel using oleaginous Chlorella J Water Process Eng. 2021;42:102183. doi: 10.1016/j.jwpe.2021.102183.
  22. Andreotti V, Solimeno A, Rossi S, Ficara E, Marazzi F, Mezzanotte V, et al. Bioremediation of aquaculture wastewater with the microalgae Tetraselmis suecica: semi-continuous experiments, simulation and photo-respirometric tests. Sci Total Environ. 2020;738:139859. doi: 1016/j.scitotenv.2020.139859.
  23. Tambaru R, Samawi MF, Afriliyeni NS. Levels of water fertility in coastal waters of Kuri based on phytoplankton chlorophyll-a concentration. IOP Conf Ser Earth Environ Sci. 2021;681(1):012106. doi: 1088/1755-1315/681/1/012106.
  24. Ning M, Li H, Xu Z, Chen L, He Y. Picophytoplankton identification by flow cytometry and high-throughput sequencing in a clean reservoir. Ecotoxicol Environ Saf. 2021;216:112216. doi: 1016/j.ecoenv.2021.112216.
  25. Lu H, Zhao R, Wang C, Zhang G, Chen C, Li B, et al. Exploration of flashing light interaction effect on improving biomass, protein, and pigments production in photosynthetic bacteria wastewater treatment. J Clean Prod. 2022;348:131304. doi: 1016/j.jclepro.2022.131304.
  26. Herold C, Ishika T, Nwoba EG, Tait S, Ward A, Moheimani NR. Biomass production of marine microalga Tetraselmis suecica using biogas and wastewater as nutrients. Biomass Bioenergy. 2021;145:105945. doi: 1016/j.biombioe.2020.105945.
  27. de Souza Leite L, Hoffmann MT, Daniel LA. Microalgae cultivation for municipal and piggery wastewater treatment in Brazil. J Water Process Eng. 2019;31:100821. doi: 1016/j. jwpe.2019.100821.
  28. Zheng XN, Wu DX, Huang CQ, Wu QY, Guan YT. Impacts of hydraulic retention time and inflow water quality on algal growth in a shallow lake supplied with reclaimed water. Water Cycle. 2022;3:71-8. doi: 1016/j.watcyc.2022.04.004.
  29. Patrinou V, Daskalaki A, Kampantais D, Kanakis DC, Economou CN, Bokas D, et al. Optimization of cultivation conditions for Tetraselmis striata and biomass quality evaluation for fish feed production. Water. 2022;14(19):3162. doi: 3390/ w14193162.
  30. Hu B, Zhou J, Dong J, Yang H, Yu G, Hong Y. Association of algae diversity and Hyriopsis schlegelii growth in mixed fish-mussel aquaculture. Algal Res. 2022;65:102736. doi: 1016/j.algal.2022.102736.
  31. Chaboki MJ, Najafpoor AA, Bonyadi Z, Dehghan A. Efficiency investigation of Chlorella vulgaris algae in removal of nitrogen, phosphorus and chemical oxygen demand from municipal and industrial wastewaters. J Res Environ Health. 2022;8(1):59-67. doi: 22038/jreh.2022.55826.1403. [Persian].

 

 

Journal of Advances in Environmental Health Research
Volume 12, Issue 1
Winter 2024
Pages 8-15