Assessment of Antibiotic Residues in Raw and Pasteurized Milk Produced by Different Analytical Methods

Document Type : Original Article


1 Department of Environmental Health Engineering, Neyshabur University of Medical Sciences, Neyshabur, Iran

2 School of Life and Environmental Science, Deakin University, Geelong, Australia

3 Responsible for Coordinating Research and Development, Veterinary Organization, Khorasan-Razavi, Mashhad, Iran

4 Student Research Committee, Neyshabur University of Medical Sciences, Neyshabur, Iran

5 Department of Food Science and Technology, Neyshabur University of Medical Sciences, Neyshabur, Iran



Background: Due to lack of attention to the required time of emulating the absorbed doses of drugs from different organs, inappropriate use of antibiotics for disease treatment in animals causes many adverse health effects in consumers. In this study, the antibiotic residues in raw and pasteurized milk were evaluated in Neyshabour city.
Methods: Raw and pasteurized milk samples were collected from eight farms and milk collection units and four pasteurized milk production factories in Neyshabour city during the warm and cold seasons. 56 samples of raw milk and 24 samples of pasteurized milk were analyzed using the Copan milk test, Tri-Sensor kit and ELISA detection methods to evaluate presence of the antibiotic residue.
Results: Copan milk test and Tri-Sensor kit did not detect the antibiotic residue. ELISA kit was able to detect the presence of gentamicin and streptomycin in milk. 25% of the samples contained gentamicin, while 10% of the samples contained streptomycin. The amount of antibiotic residue in the warm season was higher than cold season. 58.3% of pasteurized milk samples in the warm season had gentamicin.
Conclusion: Based on our results, extensive and accurate monitoring of antibiotics in raw and pasteurized milk needs to be performed in the city, especially in warm seasons. 


Main Subjects

  1. Molla Abaszadeh H, Haji Sheikhzadeh B. Surveying the contamination rate, sensibility and antimicrobial resistance patterns in Staphylococcus aureus isolated from traditional cheese consumed in Qotur of Khoy province. J Fasa Univ Med Sci. 2014;4(2):209-17. [Persian].
  2. Mahmoudi R, Norian R, Gajabeygi P. Survey of antibiotic residues in raw milk samples in Qazvin (2012). J Qazvin Univ Med Sci. 2014;18(1):45-52. [Persian].
  3. Mahmoudi R, Asadpour R, Pajohi-Alamoti MR, Golchin A, Kiyani R, Mohammad Pour R, et al. Raw cow milk quality: relationship between antibiotic residue and somatic cell count. Int Food Res J. 2013;20(6):3347-50.
  4. Ghanavi Z, Mollayi S, Eslami Z. Comparison between the amount of penicillin G residue in raw and pasteurized milk in Iran. Jundishapur J Microbiol. 2013;6(7):e12724. doi: 5812/jjm.12724.
  5. Popelka P, Nagy J, Popelka P, Marcincak S, Rozanska H, Sokol J. Comparison of sensitivity of various screening assays and liquid chromatography technique for penicillin residue detection in milk. Bull Vet Inst Pulawy. 2004;48(3):273-6.
  6. Fallah-Rad AH, Mohsenzadeh M, Assadpour HR. Determination of gentamicin residues in the raw milk delivered to Mashhad pasteurization plant and in the pasteurized milk obtained from the same raw milk. J Agric Sci Technol. 2006;7(127):183-9. [Persian].
  7. Dabbagh Moghaddam A, Tayebi L, Falahatpisheh H, Mahmoudian M, Kowsari N, Akbarein H, et al. Evaluation of the tetracycline residues in pasteurized milks distributed in Tehran by HPLC method. Ann Mil Health Sci Res. 2014;11(4):318-23. [Persian].
  8. Castiglioni S, Calamari D, Bagnati R, Zuccato E, Fanelli R. Comparison of the Concentrations of Pharmaceuticals in STPs and Rivers in Italy as a Tool for Investigating Their Environmental Distribution and Fate. Abstract SETAC Europe. In: 14th Annual Meeting; 2004.
  9. Karim G, Kiaei SM, Rokni N, Razavi Rouhani SM. Antibiotic residue contamination in milk during last forty years in Iran. Food Hygiene. 2011;1(1):23-30. [Persian].
  10. Mahmoudi R, Golchin A, Farhoodi A. A review on antibiotic residues in animal-derived foods in Iran over the last thirty years. J Mazandaran Univ Med Sci. 2014;24(119):213-22. [Persian].
  11. Mousavi SM, Jahed-Khaniki G, Rezaie S, Eskandari S, Shahsavari-Moghaddam S, Abbasi Fesarani M, et al. Evaluation of some screening tests for detection of β-lactams residues in milk production chain. J Food Saf Hyg. 2015;1(1):22-25.
  12. Popelka P, Nagy J, Popelka P, Sokol J, Hajurka J, Cabadaj R, et al. Comparison of various methods for penicillin residue detection in cow milk after intramammary and parenteral treatment. Bull Vet Inst Pulawy. 2003;47(1):203-10.
  13. Manafi M, Hesari J, Rafat SA. Monitoring of antibiotic residue in raw and pasteurized milk in East Azerbaijan of Iran by Delvotest method. J Food Res. 2010;20(2):125-31. [Persian].
  14. Zeghilet N, Bouchoucha B, Bouaziz O. βeta-lactam and tetracycline antibiotic residues in cow milk in the Constantine region, Algeria. Vet Stanica. 2022;53(3):305-11. doi: 46419/vs.53.3.8.
  15. Gaouar ZL, Loukaf K, Masmi N. Antibiotic residues in raw cow’s milk: evaluation of the situation in West Algerian region. J Fac Med Oran. 2021;5(1):653-60.
  16. Ndungu TW, Omwamba M, Muliro PS. Evaluation of rapid beta-lactam antibiotic residues detection kits for raw milk. Afr J Food Sci. 2021;15(11):353-9. doi: 5897/ajfs2021.2145.
  17. Al-Shaalan NH, Nasr JJ, Shalan S, El-Mahdy AM. Inspection of antimicrobial remains in bovine milk in Egypt and Saudi Arabia employing a bacteriological test kit and HPLC-MS/MS with estimation of risk to human health. PLoS One. 2022;17(4):e0267717. doi: 1371/journal.pone.0267717.
  18. Raza MA, Durrani AZ, Saleem MH, Ashraf K, Ali M, Akhtar KH, et al. Detection of antibiotic residues of penicillin and oxytetracycline in milk. Punjab Univ J Zool. 2022;37(1):41-8. doi: 17582/journal.pujz/2022.
  19. Kumar A, Panda AK, Sharma N. Determination of antibiotic residues in bovine milk by HPLC-DAD and assessment of human health risks in Northwestern Himalayan region, India. J Food Sci Technol. 2022;59(1):95-104. doi: 1007/s13197-021-04988-8.
  20. Igualada C, Giraldo J, Font G, Yusà V. Validation of a multi-residue UHPLC-HRMS method for antibiotics screening in milk, fresh cheese, and whey. J Food Compost Anal. 2022;106:104265. doi: 1016/j.jfca.2021.104265.
  21. Widiastuti R, Anastasia Y. Determination of tetracycline residues in dairy milk powder and fresh dairy milk by HPLC method. IOP Conf Ser Earth Environ Sci. 2022;1001(1):012039. doi: 1088/1755-1315/1001/1/012039.
  22. Bahramian B, Alizadeh Sani M, Parsa-Kondelaji M, Hosseini H, Khaledian Y, Rezaie M. Antibiotic residues in raw and pasteurized milk in Iran: a systematic review and meta-analysis. AIMS Agric Food. 2022;7(3):500-19. doi: 3934/agrfood.2022031.
  23. Mohammadzadeh Moghadam M, Amiri M, Ramezani Awal Riabi H, Ramezani Awal Riabi H. Evaluation of antibiotic residues in pasteurized and raw milk distributed in the South of Khorasan-e Razavi province, Iran. J Clin Diagn Res. 2016;10(12):FC31-FC5. doi: 7860/jcdr/2016/21034.9034.
  24. Movassagh M. Study of antibiotics residues in cow raw milk by Copan milk test in Parsabad region, Ardabil province, Iran. Ann Biol Res. 2011;2(4):355-9.
  25. Haasnoot W, Stouten P, Cazemier G, Lommen A, Nouws JF, Keukens HJ. Immunochemical detection of aminoglycosides in milk and kidney. Analyst. 1999;124(3):301-5. doi: 1039/a807846g.
  26. Joubrane K, Jammoul A, Daher R, Ayoub S, El Jed M, Hneino M, et al. Microbiological contamination, antimicrobial residues, and antimicrobial resistance in raw bovine milk in Lebanon. Int Dairy J. 2022;134:105455. doi: 1016/j.idairyj.2022.105455.
  27. Abedi Shirazi K. A survey on antibiotic contamination in milk supplied in Shiraz and its effect on public health. Dissertation. The University of Shiraz; 1984.
  28. Mansouri Nagand L. Survey on the Raw Milk Contamination with Antibiotic Residues in Kerman City. Yazd: Fourth National Symposium of Environmental Health; 2001. [Persian].
  29. Rassouli A, Abdolmaleki Z, Bokaee S, Kamkar A, Shams GR. A cross-sectional study on oxytetracycline and tetracycline residues in pasteurized milk supplied in Tehran by an HPLC method. Int J Vet Res. 2010;4(1):1-3.
  30. Ghanavi Z. Determination of penicillin G residue in raw and pasteurized milk from Tehran dairy industries dairy plant. Qazvin Univ Med Sci J. 2003;22:13-8. [Persian].