Journal of Advances in Environmental Health Research

Journal of Advances in Environmental Health Research

Antimicrobial effects of Glycyrrhiza glabra extract, iron oxide nanoparticles, and Lactobacillus rhamnosus on a biofilm composed of Pseudomonas aeruginosa in glass, wood, and polysteell

Document Type : Original Article

Authors
Masoomeh Emami
Zahra Hojjati Bonab
Department of Microbiology, Faculty of Basic Sciences, Bonab Islamic Azad University, Bonab, Iran
10.22102/jaehr.2021.250316.1185
Abstract
Biofilm formation is a pathogenicity factor of Pseudomonas aeruginosa, which causes inherent resistance to a wide range of antibiotics in the strains. The present study aimed to compare the inhibitory effects of Glycyrrhiza glabra (licorice) extract, iron oxide nanoparticles, and Lactobacillus rhamnosus suspension on a biofilm composed of P. aeruginosa in various levels of glass, wood, and polysteel. This descriptive, cross-sectional study assessed the effects of Glycyrrhiza glabra extract, iron oxide nanoparticles, and Lactobacillus rhamnosus suspension on the standard biofilm of P. aeruginosa 1601PTCC on glass, steel, and wood surfaces. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also calculated. The obtained results showed that each antimicrobial agent had different effects on P. aeruginosa, and the MIC and MBC exerted inhibitory properties. In addition, the largest inhibition zone diameter was 28 mL due to the effect of the Glycyrrhiza glabra extract on free bacteria in the volume of 180 microliters, and the highest inhibitory level was observed on the polysteel and glass surfaces with the inhibition zone diameter of 20-20.66 millimeters in the volume of 180 microliters. The highest inhibition in the bacterial biofilm was observed on the polysteel surface, and a significant difference was also denoted in this regard with the glass and wood surfaces (p <0.05). Therefore, it could be concluded that licorice (Glycyrrhiza glabra L.) had more significant antimicrobial properties compared to the iron oxide nanoparticles and Lactobacillus rhamnosus suspension.
Keywords
Pseudomonas aeruginosa
Biofilm
Iron oxide nanoparticles
Glycyrrhiza glabra extract
Lactobacillus rhamnosus
  1.  Samarghandi MR, Dargahi A, Khamutian R, Vaziri Y, Moradi-Golrokhi M. Antibiotic resistance of Escherichia coli isolated from municipal sewage in sewage treatment plant of the city of Hamadan in 2017. J Kermanshah Univ Med Sci 2018; 22(2): e79464.
  2. Shokoohi R, Samarghandi M, Dargahi A, Alikhani MY, Roshanaei G, Moradi Golrokhi M. Investigation of antibiotic resistance pattern of bacteria causing the urinary tract infection in urine samples of patients admitted in and referred to Shahid Beheshti Hospital in Hamadan. Pajouhan Sci J 2019; 17(3): 34-40. [In Persian]
  3. Johanson LS. Integrating global health concepts into study abroad curricula. Nursing 2017; 47(3): 17-8.
  4. Feldman C, Kassel M, Cantrell J, Kaka S, Morar R, Mahomed AG, et al. The presence and sequence of endotracheal tube colonization in patients undergoing mechanical ventilation. Eur Respir J 1999; 13(3): 546-51.
  5. Stepanović S, Vuković D, Ježek P, Pavlović M, Švabic-Vlahović M. Influence of dynamic conditions on biofilm formation by staphylococci. Eur J Clin Microbiol Infect Dis 2001; 20(7): 502-4.
  6. Yang G, Xie J, Hong F, Cao Z, Yang X. Antimicrobial activity of silver nanoparticle impregnated bacterial cellulose membrane: effect of fermentation carbon sources of bacterial cellulose. Carbohydr Polym 2012; 87(1): 839-45.
  7. Hemeg HA. Nanomaterials for alternative antibacterial therapy. Int J Nanomed 2017; 12: 8211-25.
  8. Slavin YN, Asnis J, Häfeli UO, Bach H. Metal nanoparticles: Understanding the mechanisms behind antibacterial activity. J Nanobiotechnol 2017; 15(1): 1-20.
  9. Wang L, Hu C, Shao L. The antimicrobial activity of nanoparticles: present situation and prospects for the future. Int J Nanomed 2017; 12: 1227-49.
  10. Torabi zarchi M, Mirhosseini M. Investigation of combination effect of magnesium oxide and iron oxide nanoparticles on the growth and morphology of the bacteria Staphylococcus aureus and Escherichia coli in juice. J Shahid Sadoughi Univ 2017; 24(11): 924-37. [In Persian]
  11. Salahvarzan A, Abdolahpour F, Ismaeili A, Sepahvand F. Anti-oxidant and anti-microbial activities of two types of honey by change in bees, diet in comparison with other honey productis in Abestan region of Khorramabad province. Yafte 2015; 17(3): 115-25. [In Persian]
  12. Stanton C, Gardiner G, Meehan H, Collins K, Fitzgerald G, Lynch PB, et al. Market potential for probiotics. Am J Clin Nutr 2001; 73(2): 476s-83s.
  13. Gharbi Y, Fhoula I, Ruas-Madiedo P, Afef N, Boudabous A, Gueimonde M, et al. In-vitro characterization of potentially probiotic Lactobacillus strains isolated from human microbiota: interaction with pathogenic bacteria and the enteric cell line HT29. Ann Microbiol 2019; 69(1): 61-72.
  14. Hojjati Bonab Z, Nikkhah E. Evaluation of antioxidant and antibacterial effect of methanolic extract from thyme (Thymus vulgar), senna (Cassia angustifolia) and licorice (Glycyrrhiza glabra). Daneshvar 2012; 19(3): 57-66. [In Persian]
  15. Sadri M, Arbabsoleimani N, Forghanifard MM. The study of antimicrobial and anti-adhesive effect of probiotic Lactobacilli on Uropathogenic Escherichia coli (UPEC). Biol J Microorgan 2016; 5(17): 159-7 .[In Persian]
  16. Mahdavi MA, Jalali MA. Biofilm formation of Listeria monocytogenes on various surfaces. Armaghane- Danesh 2007; 12(3): 47-57. [In Persian]
  17. Chakotiya AS, Tanwar A, Narula A, Sharma RK. Alternative to antibiotics against Pseudomonas aeruginosa: Effects of Glycyrrhiza glabra on membrane permeability and inhibition of efflux activity and biofilm formation in Pseudomonas aeruginosa and its in vitro time-kill activity. Microb Pathog 2016; 98: 98-105.
  18. Rahimzadeh G, Bahar MA, Amir Mozafari N, Salehi M. Antimicrobial activity Kefir on Pseudomonas aeruginosa. Razi J Med Sci 2011; 18(82 & 83): 8-16. [In Persian]
  19. Ghotaslou R, Salahi Eshlaqghi B. Biofilm of pseudomonas aeruginosa and new preventive measures and anti-biofilm agents. J Rafsanjan Univ Med Sci 2013; 12(9): 747-68. [In Persian]
  20. Abdi M, Soltan Dallal MM, Hajiabdolbaghi M, Douraghi M, Davoodabadi A. Study of antagonistic properties of lactobacilli isolated from healthy baby stools on growth of Acinetobacter baumannii and Pseudomonas aeruginosa of nosocomial origin. Pajoohandeh 2016; 20(6): 302-7. [In Persian]
  21. Ramezani Ali Akbari K, Ahya Abdi A. Study of antimicrobial effects of several antibiotics and iron oxide nanoparticles on biofilm producing pseudomonas aeruginosa. Nanomed J 2017; 4(1): 37-43.
Journal of Advances in Environmental Health Research
Volume 8, Issue 4
Autumn 2020
Pages 260-268