Evaluation of Antibacterial Effect of Aqueous, Hydro-alcoholic and Alcoholic Extracts of Morus nigra on Gram-positive and Gram-negative Bacteria

Document Type : Original Article


1 Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran.

2 Department of Chemistry, Rasht Branch, Islamic Azad University, Rasht, Iran.



Background: Microbial resistance to antibiotics has led to serious efforts to discover newer drugs, which is why there is so much interest in the use of herbs. Therefore, the aim of this study was to investigate the antibacterial effect of aqueous, hydro-alcoholic, and alcoholic extracts of Morus nigra as well as some common antibiotics by disk diffusion method.
Methods: In this experimental study, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the extracts were determined using the macro dilution method. Statistical analysis was performed using the ANOVA test by SPSS software.
Results: The results of this study showed that all strains studied were sensitive to different extracts of Morus nigra, but the most effective effect of aqueous extract of Morus nigra against Streptococcus genus bacteria with a growth inhibition zone of 22.25 mm. As this effect was higher than penicillin, gentamicin, and tetracycline antibiotics, it was not statistically different for cefixime. MIC values ​​were between 0.78 and 3.12 mg/mL and MBC values ​​were between 3.12 and 50 mg/mL.
Conclusion: Based on the findings of this study, the aqueous extract of Morus nigra has shown strong bactericidal properties against all bacterial strains studied here, which indicates its effective therapeutic application.


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