Improved removal of Trinitrotoluene (TNT) from contaminated soil by inducing aerobic process: kinetic and chemical byproducts

Document Type: Original Article

Authors

1 Nutrition Health Research Center, Department of Environment Health, School of Health, Lorestan Medical Sciences University, Khoramabad, Iran.

2 Nutrition Health Research Center, Department of Environment Health, School of Health, Lorestan Medical Sciences University, Khoramabad, Iran

3 Department of chemistry, Tehran medical sciences branch, IslamicAzad university, Tehran,

Abstract

This study describes the biological degradation of TNT by using induced aeration. Three plastic reactors were used. In each reactor 3 kg of soil were used. In order to increase the porosity of the soil, sawdust was added to soil. Textile wastewater treatment plant sludge was also added to soil. TNT at the concentrations of 1000 mg/kg of soil was added thereafter. Rhamnolipid biosurfactant at the concentration of 60 mg/L was added to related reactors. Aeration interval was at every 3 to 5 days. Every two weeks, sampling of soil were done to analyze the explosives. Samples were analyzed by HPLC. The results showed that at the end of 120 days, TNT removal efficiency in induced aeration in reactors containing sludge and biosurfactant was 98 percent. COD removal efficiency in induced aeration in reactors amended by rhamnolipid was 58 percent and in reactors to which rhamnolipid was not added was 41 percent. Follow-up kinetic studies revealed that explosives removal follow the pseudo first order reaction. The pseudo first-order rate constants of rhamnolipid amended experiments were at least 3.89 orders of magnitude higher for TNT than those found for experiments without rhamnolipid. Application of Rhamnolipid biosurfactant could have a protective effect against the toxicity of explosives for bacteria. Textile sludge from wastewater treatment plant can decrease the time needed for explosive removal. Growth of bacteria and degradation of explosives showed that explosives have been used as a nitrogen source. 

Keywords

References

  1. Singh A, Kuhad R C, Ward P. Advances in Applied Bioremediation: Springer Dordrecht Heidelberg London New York 2009; 14-18.
  2. Meyers S, Shanley ES. Industrial explosives - a brief history of their development and use. Journal of Hazardous Materials .1990; 23(2): 183-201.
  3. Rosenblatt DH, Burrows  EP,  Mitchell  W.R.  ,  Parmer,  D.L .Organic explosives and related compounds, In O. Hutzinger, The handbook of environmental chemistry.Berlin: Springer- Verlag,1991.
  4. Sheibani G, Naeimpoor F, Hejazi P. Statistical factor-screening and optimization in slurry phase bioremediation of 2,4,6 -trinitrotoluene contaminated soil. Journal of Hazardous Materials. 2011; 188 (1-3): 1-9.
  5. Pennington JC,Barnnon  JM. Environmental fate of explosives. Thermochemica Act. 2002; 384(1-2): 163-172.
  6. Widrig DL, Boopathy RJ, Manning JR. Bioremediation of TNT- contaminanted soil: a laboratory study. Environmental Toxicology and Chemistry. 1997; 16(6): 1141-1148.
  7. Clark B, Boopathy R. Evaluation of bioremediation methods for the treatment of soil contaminated with explosives in Louisiana Army Ammunition Plant, Minden, Louisiana. Journal of Hazardous Materials.2007; 143(3): 643-648.
  8. Boopathy R. Effect of food-grade surfactant on bioremediation of explosives-contaminated soil. Journal of Hazardous Materials 2002; 92(1): 103-114.
  9. Zhuang L, Gui L, Gillham RW. Biodegradation of pentaerythritol tetranitrate (PETN) by anaerobic consortia from a contaminated site. Chemosphere.  2012; 89(7): 810-816.
  10. Chrzanowski Ł, Owsianiak M, Szulc A, Marecik R, Piotrowska-Cyplik A, Olejnik-Schmidt AK, et al. Interactions between rhamnolipid biosurfactants and toxic chlorinated phenols enhance biodegradation of a model hydrocarbon-rich effluent. International Biodeterioration & Biodegradation. 2011; 31;65(4):605-11.
  11. Avramova T, Sotirova A, Galabova D, Karpenko E. Effect of Triton X-100 and rhamnolipid PS-17 on the mineralization of phenanthrene by Pseudomonas sp. cells. International Biodeterioration & Biodegradation. 2008;62(4):415-20.
  12. Christodoulatos C, Bhaumik  S,  Brodman  BW. Anaerobic biodegradation of nitroglycerin. Water Research. 1997; 31(6): 1462-1470.
  13. Meng  M, Sun W,  Geelhaar LA,  Kumar G,  Patel AR,  Payne GF, et al. Denitration of glycerol trinitrate by resting cells and cell extracts of Bacillus thuringiensis/cereus and Enterobacter agglomerans. Applied and Environmental Microbiology. 1995;61(7): 2548-2553.
  14. Innemanová P, Velebová R, Filipová A, Čvančarová M, Pokorný P, Němeček J, et al. Anaerobic in situ biodegradation of TNT using whey as an electron donor: a case study. New biotechnology. 2015;32(6):701-9.
  15. Mazaheri Assadi M, Tabatabaee MS. Biosurfactants and their use in upgrading petroleum vacuum distillation residue: A review. International Journal of Environmental Research. 2010; 4(4): 549-572.
  16. Numbera AA. Method 8330B nitroaromatics, nitramines, and nitrate esters by high performance liquid chromatography (HPLC). US Environmental Protection Agency, Washington, DC. 2006.
  17. Federation WE, American Public Health Association. Standard methods for the examination of water and wastewater. American Public Health Association (APHA): Washington, DC, USA. 2005.
  18. Son A, Lee J, Chiu PC, Kim BJ, Cha DK. Microbial reduction of perchlorate with zero-valent iron. Water research. 2006;40(10):2027-32.
  19. Lewis TA, Newcombe DA, Crawford RL. Bioremediation of soils contaminated with explosives. Journal of Environmental Management. 2004;70(4):291-307.
  20. Moussavi G, Aghapour AA, Yaghmaeian K. The degradation and mineralization of catechol using ozonation catalyzed with MgO/GAC composite in a fluidized bed reactor. Chemical Engineering Journal. 2014;249:302-310.
  21. Sponza DT, Gök O. Effect of rhamnolipid on the aerobic removal of polyaromatic hydrocarbons (PAHs) and COD components from petrochemical wastewater. Bioresource technology. 2010;101(3):914-924.
  22. Lin HY, Yu CP, Chen ZL. Aerobic and anaerobic biodegradation of TNT by newly isolated Bacillus mycoides. Ecological engineering. 2013;52:270-277.
  23. Montpas S, Samson J, Langlois É, Lei J, Piché Y, Chênevert R. Degradation of 2, 4, 6-trinitrotoluene by Serratia marcescens. Biotechnology letters. 1997;19(3):291-294.
  24. Nyanhongo GS, Schroeder M, Steiner W, Gübitz GM. Biodegradation of 2, 4, 6-trinitrotoluene (TNT): An enzymatic perspective. Biocatalysis and Biotransformation. 2005;23(2):53-69.
Journal of Advances in Environmental Health Research

Volume 5, Issue 3
Summer 2017
Pages 139-145

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