Biodegradation of methylene blue from aqueous solution by bacteria isolated from contaminated soil

Document Type : Original Article

Authors

1 PhD Student of Environmental Health, Environmental Science and Technology Research Center, School of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

2 Assistant professor, Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran

3 MSc of Microbiology, Laboratory of Microbiology, School of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

4 Lecturer, Department of Environmental Health Engineering ,Gonabad University of Medical Sciences, Gonabad, Iran

5 Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran

Abstract

The use of biodegradation methods by microorganisms in the removal of industrial dyes are widely considered owing to their high efficiency and compatibility to the environment. Therefore, this study aims to evaluate the biodegradation of methylene blue (MB) from aqueous solution by bacteria isolated from contaminated soil. This study was performed in laboratory scale on bacteria isolated and purified from contaminated soil with methylene blue. Initially, the bacteria was isolated from contaminated soil. Thereafter, medium containing 50, 100, 200, 400, 800 and 1000 mg/l of methylene blue, 50 ml of salt medium with glucose and 2.5 ml of Brain-heart infusion (BHI) medium containing bacteria were prepared. The results of dye removal were analyzed using UV/Vis spectrophotometer at 665 nm. The results of purification and identification of the bacterial species which degrade methylene blue indicated that Pseudomonas aeruginosa was the dominant bacteria. In this study, the removal efficiency of bacteria was attained from 82.25 to 97.82% with an increase in initial MB concentration from 50 to 200 mg/l. Nevertheless, with increase in MB  oncentration from 200 to 1000 mg/l, removal efficiency was reduced to 43.08%. The optimum concentration of MB removal was 200 mg/l. It is evident from the results that the bacteria had used methylene blue as an auxiliary source of carbon apart from glucose. Finally, it can be concluded that P. aeruginosa is an appropriate candidate for the removal of methylene blue from the environment.

Keywords

References

  1. Shahi DH, Eslami H, Ehrampoosh MH, Ebrahimi A, Ghaneian MT, Ayatollah S, et al. Comparing the efficiency of Cyperus alternifolius and Phragmites australis in municipal wastewater treatment by subsurface constructed wetland. Pak J Biol Sci 2013;16(8):379-384.
  2. Eslami H, Hematabadi PT, Ghelmani SV, Vaziri AS, Derakhshan Z. The Performance of Advanced Sequencing Batch Reactor in Wastewater Treatment Plant to Remove Organic Materials and Linear Alkyl Benzene Sulfonates. Jundishapur Journal of Health Sciences 2015;7(3):33-39.
  3. Xiao Y, Liu X, Wang D, Lin Y, Han Y, Wang X. Feasibility of using an innovative PVDF MF membrane prior to RO for reuse of a secondary municipal effluent. Desalination 2013;311:16-23.
  4. Yagub MT, Sen TK, Afroze S, Ang HM. Dye and its removal from aqueous solution by adsorption: a review. Adv Colloid Interface Sci 2014;209:172-184.
  5. Yao Y, Xu F, Chen M, Xu Z, Zhu Z. Adsorption behavior of methylene blue on carbon nanotubes. Bioresour Technol 2010;101(9):3040-3046.
  6. Singh RL, Singh PK, Singh RP. Enzymatic decolorization and degradation of azo dyes–A review. Int Biodeterior Biodegrad 2015;104:21-31.
  7. Ghanizadeh G, Asgari G. Removal of methylene blue dye from synthetic wastewater with bone char. Iranian Journal of Health and Environment 2009;2(2):104-13.[In persion]
  8. Yang C, Li L, Shi J, Long C, Li A. Advanced treatment of textile dyeing secondary effluent using magnetic anion exchange resin and its effect on organic fouling in subsequent RO membrane. J Hazard Mater 2015;284:50-57.
  9. Özer A, Dursun G. Removal of methylene blue from aqueous solution by dehydrated wheat bran carbon. J Hazard Mater 2007;146(1):262-9.
  10. Dos Santos AB, Cervantes FJ, van Lier JB. Review paper on current technologies for decolourisation of textile wastewaters: perspectives for anaerobic biotechnology. Bioresour Technol. 2007;98(12):2369-2385.
  11. Kalaivani GJ, Suja S. TiO2 (rutile) embedded inulin—A versatile bio-nanocomposite for photocatalytic degradation of methylene blue. Carbohydr Polym. 2016;143:51-60.
  12. Karagozoglu B, Tasdemir M, Demirbas E, Kobya M. The adsorption of basic dye (Astrazon Blue FGRL) from aqueous solutions onto sepiolite, fly ash and apricot shell activated carbon: Kinetic and equilibrium studies. J Hazard Mater 2007;147(1-2):297-306.
  13. Gupta V. Application of low-cost adsorbents for dye removal–A review. J Environ Manage. 2009;90(8):2313-42.
  14. Santos SC, Vilar VJ, Boaventura RA. Waste metal hydroxide sludge as adsorbent for a reactive dye. J Hazard Mater 2008;153(3):999-1008.
  15. Zodi S, Merzouk B, Potier O, Lapicque F, Leclerc J-P. Direct red 81 dye removal by a continuous flow electrocoagulation/flotation reactor. Sep Purif Technol 2013;108:215-22.
  16. Forgacs E, Cserhati T, Oros G. Removal of synthetic dyes from wastewaters: a review. Environ Int 2004;30(7):953-71.
  17. Gopinath KP, Murugesan S, Abraham J, Muthukumar K. Bacillus sp. mutant for improved biodegradation of Congo red: random mutagenesis approach. Bioresour Technol 2009;100(24):6295-6300.
  18. Baldev E, MubarakAli D, Ilavarasi A, Pandiaraj D, Ishack KSS, Thajuddin N. Degradation of synthetic dye, Rhodamine B to environmentally non-toxic products using microalgae. Colloids Surf B Biointerfaces 2013;105:207-14.
  19. Lv G-Y, Cheng J-H, Chen X-Y, Zhang Z-F, Fan L-F. Biological decolorization of malachite green by Deinococcus radiodurans R1. Bioresour Technol 2013;144:275-280.
  20. Tan L, Ning S, Zhang X, Shi S. Aerobic decolorization and degradation of azo dyes by growing cells of a newly isolated yeast Candida tropicalis TL-F1. Bioresour Technol 2013;138:307-313.
  21. Pearce C, Lloyd J, Guthrie J. The removal of colour from textile wastewater using whole bacterial cells: a review. Dyes and pigments 2003;58(3):179-196.
  22. Chen Z, Chen H, Pan X, Lin Z, Guan X. Investigation of methylene blue biosorption and biodegradation by Bacillus thuringiensis 016. Water, Air, Soil Pollut 2015;226(5):1-8.
  23. El-Sersy NA. Bioremediation of Methylene Blue by Bacillus thuringiensis 4 G 1: Application of Statistical Designs and Surface Plots for Optimization. Biotechnology 2007;6(1):34-39.
  24. Noraini CHC, Morad N, Norli I, Teng TT, Ogugbue CJ. Methylene blue degradation by Sphingomonas paucimobilis under aerobic conditions. Water, Air, Soil Pollut 2012;223(8):5131-5142.
  25. Sarvi Moghanlo V, Chorom M, Motamedy H, Pourzamani H, Falah M. Identification of Hydrocarbon Degrading Bacteria Genus from Oil-Crude Contaminated Soils. J Health Syst Res 2012;8(6):1098-106.[In Perstion]
  26. Kafilzadeh F, Hoshyaripour F, Afrough R, Jamali H, Allahverdi G. Isolation and Identification of Pyrene-degrading Bacteria from Soils around Landfills in Shiraz and Their Growth Kinetic Assay. Journal of Fasa University of Medical Sciences 2011;1(3):154-9. [In Perstion]
  27. Jing W, Byung-Gil J, Kyoung-Sook K, Young-Choon L, Nak-Chang S. Isolation and characterization of Pseudomonas otitidis WL-13 and its capacity to decolorize triphenylmethane dyes. J Environ Sci (China) 2009;21(7):960-4.
  28. Pandey A, Singh P, Iyengar L Bacterial decolorization and degradation of azo dyes. Int Biodeterior Biodegrad 2007;59(2):73-84.
  29. Núñez SC, Garcez AS, Kato IT, Yoshimura TM, Gomes L, Baptista MS, et al. Effects of ionic strength on the antimicrobial photodynamic efficiency of methylene blue. Photochemical & Photobiological Sciences 2014;13(3):595-602.
  30. Perni S, Piccirillo C, Pratten J, Prokopovich P, Chrzanowski W, Parkin IP, et al. The antimicrobial properties of light-activated polymers containing methylene blue and gold nanoparticles. Biomaterials 2009;30(1):89-93.
  31. Lalnunhlimi S, Krishnaswamy V. Decolorization of azo dyes (Direct Blue 151 and Direct Red 31) by moderately alkaliphilic bacterial consortium. Braz J Microbiol 2016;47(1):39-46.
  32. Karatay SE, Kılıç NK, Dönmez G. Removal of Remazol Blue by azoreductase from newly isolated bacteria. Ecol Eng 2015;84:301-4.

 

Journal of Advances in Environmental Health Research
Volume 5, Issue 1
February 2017
Pages 10-15

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