Effective AFM1 determination in ice cream in seven Iranian cities: An empirical study based on immunoaffinity column clean-up followed by HPLC-FLD

Document Type : Original Article

Authors

1 Department of Chemistry, Islamic Azad University, Tabriz Branch, Tabriz, Iran.

2 Department of Chemistry, Islamic Azad University, Tehran South Branch, Tehran, Iran.

3 Department of Chemistry, Islamic Azad University, Sanandaj Branch, Sanandaj, Iran.

10.22102/jaehr.2022.321596.1264

Abstract

Aflatoxin M1 (AFM1) in industrial ice cream was analyzed by immunoaffinity column (IAC) clean-up followed by high-performance liquid chromatography (HPLC) with a fluorescence detector (FLD) as a highly-sensitive method to confirm and quantify AFM1 in ice cream samples. A total of 150 (industrial) ice cream samples was randomly collected from supermarkets of seven Iranian cities (Tehran, Tabriz, Karaj, Urmia, Sanandaj, Qazvin, and Khoramabad). AFM1 was detected in more than 80.0% of samples. The average AFM1 concentration in ice cream was 29.79 ng L-1. According to the results, in 12.62% of samples, AFM1 levels exceeded the maximum EU limits for adults (50 ng mL-1). This study recommends regular monitoring of AFM1 concentration in ice cream in Iran. Results of this study indicate the need for creation a database to assist law enforcement agencies, traders, and policymakers in local governments to minimize or prevent AFM1-induced health-related risks.

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