TY - JOUR ID - 57889 TI - Chromium bioremediation by Alcaligenes sp. strain newly isolated from chromite mine of Sabzevar JO - Journal of Advances in Environmental Health Research JA - JAEHR LA - en SN - 2676-3478 AU - Mesbahi-Nowrouzi, Mohsen AU - Mollania, Nasrin AU - Khajeh, Khosro AD - Department of Biology, Faculty of Basic Sciences, Hakim Sabzevari University, Sabzevar, Iran AD - Department of Biochemistry and Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Y1 - 2017 PY - 2017 VL - 5 IS - 4 SP - 220 EP - 232 KW - Bioremediation KW - Carcinogenic Cr(VI) KW - Chromium reduction KW - Reductase KW - Response Surface Methodology DO - 10.22102/jaehr.2018.82265.1014 N2 - In this work, CKCr-6A strain was found to be highly resistant to some toxic heavy metals such as Cr+6, Cr+3, Cu+2, Co+2, Cd+2, Pb+2, Hg+2, U+6, tellurium, and selenite. Herein, high chromate tolerance of an isolated strain is reported with a high minimum inhibitory concentration value of 80,000 mg/L and the effective parameters (pH, temperature, shaking, and glucose concentration) were selected for Cr(VI) removal by this isolated strain. Cr(VI) elimination by the target strain increased with glucose addition to the culture medium. We optimized the possible parameters and their interactions using design experimental software. After optimization, this strain showed high efficiency in detoxifying chromate; this could reduce up to 100 mg/L of Cr(VI) to Cr(III) over 3 h. The CKCr-6A strain exhibited ability to in vitro reduction after 3 h and repeated removing of Cr(VI) without any amendment of nutrients, suggesting its possible application in continuous bioremediation. UR - https://jaehr.muk.ac.ir/article_57889.html L1 - https://jaehr.muk.ac.ir/article_57889_fc944c2a60e08624ac8ce4d0bdf1b412.pdf ER -